Peripheral nerve injury is the most devastating trauma of hand in the clinical practice, especially when critical nerve gap encountered. Various advanced therapies have beneficial effects promoting peripheral nerve regeneration. Our NMR lab devotes modern multidisciplinary approaches by biomimetic biomaterials, stem cell therapy and electroceuticals as treatment modalities to promote NeuroMuscular Regeneration.
Proximal electrical stimulation (PES) (upstream electric current to spinal cord) has shown positive effects on peripheral nerve regeneration by Tessa Gordon group and the therapeutic benefit is well-established in animal models and human trials . On the other hand, distal electrical stimulation (DES)(downstream electric current to innervated muscle) has demonstrated protective benefit to recapitulate physiologic electrical impulse and prevent robust neuromuscular junction atrophy, leading to better neuromuscular reinnervation. Our lab aims to investigate the effects of neuromodulation in peripheral nerve injury with clinical relevant nerve injury animal models.
There are multiple studies using ADSCs in a variety of animal models to improve the neurological recovery. The ADSCs release a range of neurotrophic factors, such as EGF, TGFβ-1, VEGF, bFGF, HGF, IGF-I, and BDNF at different stages of tissue regeneration which are also thought to promote the healing of the damaged peripheral nerve. Moreover, the additional immunomodulatory regulation of ADSCs also contributes to ameliorate sustained neuroinflammation, resulting in Macrophage transition and peripheral nerve regeneration.
Compressive neuropathy of the upper extremity, including carpal and cubital tunnel syndromes, is the most common disease for hand surgeons. According to the previous studies, two pathological mechanisms including (1) mechanical compression and (2) ischemia were believed to be involved in the compressive neuropathy. The surgical decompression was the gold standard treatment but most patients still felt unsatisfied after surgery. We focus on nerve ischemia and over-inflammation to induce peripheral neuropathy and try to develop the optimal mechanical base treatment of peripheral neuropathy by stem cells therapy or electrical stimulation.
- Szu-Han Chen , Chia-Ching Wu, Wan-Ling Tseng, Fu-I Lu, Ya-Hsin Liu, Shau-Ping Lin, Sheng-Che Lin , Yuan-Yu Hsueh*. Adipose-derived stem cells modulate neuroinflammation and improve functional recovery in chronic constriction injury of the rat sciatic nerve. Front Neurosci. 2023 Jun 29;17:1172740
- Ming-Wei Hsu, Szu-Han Chen, Wan-Ling Tseng, Kuo-Shu Hung, Tzu-Chun Chung, Sheng-Che Lin, Jahyun Koo, Yuan-Yu Hsueh*. Physical processing for decellularized nerve xenograft in peripheral nerve regeneration. Front. Bioeng. Biotechnol. 2023 May 30; 11:1217067. doi: 10.3389/fbioe.2023.1217067
- Woo-Youl Maeng, Wan-Ling Tseng, Song Li, Jahyun Koo, Yuan-Yu Hsueh*. Electroceuticals for peripheral nerve regeneration. Biofabrication 2022 Sep;14(4): 042002-042021
- Szu-Han Chen, Chia-Ching Wu, Sheng-Che Lin, Wan-Ling Tseng, Tzu-Chieh Huang, Anjali Yadav, Fu-I Lu, Ya-Hsin Liu, Shau-Ping Lin, Yuan-Yu Hsueh* Investigation of neuropathology after nerve release in chronic constriction injury of rat sciatic nerve. International Journal of Molecular Sciences 2021; 22(9):4746-4763
- Yeon Sik Choi#, Yuan-Yu Hsueh#, Jahyun Koo#, Quansan Yang#, Buwei Hu, Zhaoqian Xie, Raudel Avila, Geumbee Lee, Ning Zheng, Claire Liu, Yameng Xu, Young Joong Lee, Weikan Zhao, Jun Fang, Yujun Deng, Seung Min Lee, Iwona Stepien, Ying Yan, Joseph Song, Chad Haney, Yong Suk Oh, Wentai Liu, Hong-Joon Yoon, Anthony Banks, Matthew MacEwan, Guillermo Ameer, Wilson Ray, Yonggang Huang ,Colin Franz, Tao Xie, Song Li, Abraham Vazquez-Guardado, John A. Rogers. Stretchable, dynamic covalent polymers for soft, long-lived bioresorbable electronic stimulators designed to facilitate neuromuscular regeneration. Nature Communications 2020; 11(1):5990-6003
- Chen SH, Huang TC, Wang JY, Wu CC, Hsueh YY*. Controllable forces for reproducible chronic constriction injury mimicking compressive neuropathy in rat sciatic nerve. J Neurosci Methods. 2020 Apr; 335:108615.
- Fang J, Hsueh YY, Soto J, Sun W, Wang J, Gu Z, Khademhosseini A, Li S. Engineering Biomaterials with Micro/Nanotechnologies for Cell Reprogramming. ACS Nano. 2020 Feb; 14(2):1296-1318.
- Hsueh YY, Wang DH, Huang TC, Chang YJ, Shao WC, Tuan TL, Hughes MW, Wu CC. Novel skin chamber for rat ischemic flap studies in regenerative wound repair. Stem Cell Res Ther. 2016 May; 7(1):72.
- Hsueh YY, Chang YJ, Huang CW, Handayani F, Chiang YL, Fan SC, Ho CJ, Kuo YM, Yang SH, Chen YL, Lin SC, Huang CC, Wu CC. Synergy of endothelial and neural progenitor cells from adipose-derived stem cells to preserve neurovascular structures in rat hypoxic-ischemic brain injury. Sci Rep. 2015 Oct; 5:14985.
- Hsueh YY, Chang YJ, Huang TC, Fan SC, Wang DH, Chen JJ, Wu CC, Lin SC. Functional recoveries of sciatic nerve regeneration by combining chitosan coated conduit and neurosphere cells induced from adipose-derived stem cells. Biomaterials. 2014 Feb; 35(7):2234-44.
- Hsueh YY, Chiang YL, Wu CC, Lin SC. Spheroid formation and neural induction in human adipose-dervived stem cells on a chitosan-coated surface. Cells Tissues Organs,2012; 196(2):117-128.
NCKU-NMR Lab 